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WB analysis of HepG2 cells using GTX46692 WDR33 antibody at 0.2-1microg/ml.
WB analysis of HepG2 cells using GTX46692 WDR33 antibody at 0.2-1microg/ml.
WB analysis of HepG2 cells using GTX46692 WDR33 antibody at 0.2-1microg/ml.

WDR33 antibody, Internal

GTX46692
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetWDR33
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Overview

  • Supplier
    GeneTex
  • Product Name
    WDR33 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55339
  • Target name
    WDR33
  • Target description
    WD repeat domain 33
  • Target synonyms
    NET14; pre-mRNA 3' end processing protein WDR33; WD repeat-containing protein 33; WD repeat-containing protein of 146 kDa; WD repeat-containing protein WDC146; WDC146
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0J8
  • Protein Name
    pre-mRNA 3' end processing protein WDR33
  • Scientific Description
    This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is highly expressed in testis and the protein is localized to the nucleus. This gene may play important roles in the mechanisms of cytodifferentiation and/or DNA recombination. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WDR33 using anti-WDR33 antibody (A09589-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR33 antigen affinity purified polyclonal antibody (Catalog # A09589-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR33 at approximately 170-180 kDa. The expected band size for WDR33 is at 146 kDa.
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