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Immunohistochemical staining of human Heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human Heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human Heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.

Anti-WFS1 Antibody

HPA029128
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetWFS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WFS1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7466
  • Target name
    WFS1
  • Target description
    wolframin ER transmembrane glycoprotein
  • Target synonyms
    CTRCT41, WFRS, WFS, WFSL, wolframin, Wolfram syndrome 1 (wolframin)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO76024
  • Protein Name
    Wolframin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WFS1 using anti-WFS1 antibody (A01326-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human T-47D whole cell lysates, Lane 3: monkey lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WFS1 antigen affinity purified polyclonal antibody (Catalog # A01326-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WFS1 at approximately 100KD. The expected band size for WFS1 is at 100KD.
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