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ProductsBack/Wolfram Syndrome Protein 1 (WFS1) Monoclonal Antibody

Wolfram Syndrome Protein 1 (WFS1) Monoclonal Antibody

CAU35159
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetWFS1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Wolfram Syndrome Protein 1 (WFS1) Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C8
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7466
  • Target name
    WFS1
  • Target description
    wolframin ER transmembrane glycoprotein
  • Target synonyms
    CTRCT41; WFRS; WFS; WFSL; Wolfram syndrome 1 (wolframin); wolframin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO76024
  • Protein Name
    Wolframin
  • Scientific Description
    The Wolfram Syndrome Protein 1 (WFS1) Monoclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WFS1 using anti-WFS1 antibody (A01326-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human T-47D whole cell lysates, Lane 3: monkey lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WFS1 antigen affinity purified polyclonal antibody (Catalog # A01326-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WFS1 at approximately 100KD. The expected band size for WFS1 is at 100KD.
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