Figure 1. Western blot analysis of WISP1 using anti-WISP1 antibody (PA2089). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WISP1 antigen affinity purified polyclonal antibody (Catalog # PA2089) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WISP1 at approximately 45 kDa. The expected band size for WISP1 is at 40 kDa.
Figure 1. Western blot analysis of WISP1 using anti-WISP1 antibody (PA2089). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WISP1 antigen affinity purified polyclonal antibody (Catalog # PA2089) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WISP1 at approximately 45 kDa. The expected band size for WISP1 is at 40 kDa.
Anti-WISP1 Antibody Picoband(r)
PA2089
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetCCN4
Overview
- SupplierBoster Bio
- Product NameAnti-WISP1/CCN4 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierIHP, IHF, ICC, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID8840
- Target nameCCN4
- Target descriptioncellular communication network factor 4
- Target synonymsCCN family member 4; WISP1; WISP1 3'UTR-associated RNA 1; WISP1 overlapping transcript 1 (non-protein coding); WISP1c; WISP1i; WISP1-OT1; WISP1tc; WISP1-UT1; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1
- HostRabbit
- IsotypeIgG
- Protein IDO95388
- Protein NameWNT1-inducible-signaling pathway protein 1
- Scientific DescriptionBoster Bio Anti-WISP1 Antibody catalog # PA2089. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Bovine
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203