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Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Anti-WNT5A Antibody

AMAB91883
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetWNT5A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WNT5A
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL13683
  • Conjugate
    Unconjugated
  • Gene ID7474
  • Target name
    WNT5A
  • Target description
    Wnt family member 5A
  • Target synonyms
    epididymis secretory sperm binding protein; hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP41221
  • Protein Name
    Protein Wnt-5a
  • Scientific Description
    Synthetic Peptide
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Mouse heart tissue All lanes: WNT5A antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 41 kDa Observed band size: 43 kDa
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Figure 1. Western blot analysis of WNT5A using anti-WNT5A antibody (A00549-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT5A antigen affinity purified polyclonal antibody (Catalog # A00549-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT5A at approximately 43 kDa. The expected band size for WNT5A is at 42 kDa.
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