Figure 2. Flow Cytometry analysis of PC-3 cells using anti-WNT7A antibody (A01728). Overlay histogram showing PC-3 cells stained with A01728 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-WNT7A Antibody (A01728, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 2. Flow Cytometry analysis of PC-3 cells using anti-WNT7A antibody (A01728). Overlay histogram showing PC-3 cells stained with A01728 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-WNT7A Antibody (A01728, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Anti-WNT7A Antibody Picoband(r)
A01728-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetWNT7A
Overview
- SupplierBoster Bio
- Product NameAnti-WNT7A Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID7476
- Target nameWNT7A
- Target descriptionWnt family member 7A
- Target synonymsprotein Wnt-7a; proto-oncogene Wnt7a protein; wingless-type MMTV integration site family, member 7A; Wnt-7a
- HostRabbit
- IsotypeIgG
- Protein IDO00755
- Protein NameProtein Wnt-7a
- Scientific DescriptionBoster Bio Anti-WNT7A Antibody Picoband® catalog # A01728. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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