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Immunohistochemical staining of human testis shows moderate nuclear positivity in Leydig cells.
Immunohistochemical staining of human testis shows moderate nuclear positivity in Leydig cells.
Immunohistochemical staining of human testis shows moderate nuclear positivity in Leydig cells.

Anti-WRN Antibody

HPA028661
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetWRN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WRN Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7486
  • Target name
    WRN
  • Target description
    WRN RecQ like helicase
  • Target synonyms
    RECQ3, RECQL2, RECQL3, bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN, DNA helicase, RecQ-like type 3, Werner syndrome ATP-dependent helicase, Werner syndrome RecQ like helicase, Werner syndrome protein, Werner syndrome, RecQ helicase-like, exonuclease WRN, recQ protein-like 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14191
  • Protein Name
    Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of WRN using anti-WRN antibody (PB10107). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRN antigen affinity purified polyclonal antibody (Catalog # PB10107) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WRN at approximately 162kDa, 200 kDa. The expected band size for WRN is at 162 kDa.
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Immunofluorescent staining of human cell line U2OS shows localization to nuclear speckles.
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