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Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.

Anti-WWC1 Antibody

HPA038017
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetWWC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WWC1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23286
  • Target name
    WWC1
  • Target description
    WW and C2 domain containing 1
  • Target synonyms
    HBEBP3, HBEBP36, KIBRA, MEMRYQTL, PPP1R168, protein KIBRA, HBeAg-binding protein 3, WW, C2 and coiled-coil domain containing 1, kidney and brain protein, protein WWC1, protein phosphatase 1, regulatory subunit 168
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IX03
  • Protein Name
    Protein KIBRA
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of KIBRA/WWC1 using anti-KIBRA/WWC1 antibody (A02837-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Mcf-7 whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIBRA/WWC1 antigen affinity purified polyclonal antibody (Catalog # A02837-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIBRA/WWC1 at approximately 130KD. The expected band size for KIBRA/WWC1 is at 130KD.
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Lane 1: Siha cell lysates;Lane 2: A431 cell lysates;Lane 3: MCF-7 cell lysates; Lane 4: U2OS cell lysates probed with KIBRA Polyclonal Antibody, Unconjugated (bs-11570R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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