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ProductsBack/WWC1 / KIBRA Antibody (aa847-1094)

WWC1 / KIBRA Antibody (aa847-1094)

LS-C373765
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetWWC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    WWC1 / KIBRA Antibody (aa847-1094)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:50 - 1:400) ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.43 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23286
  • Target name
    WWC1
  • Target description
    WW and C2 domain containing 1
  • Target synonyms
    HBEBP3, HBEBP36, KIBRA, MEMRYQTL, PPP1R168, protein KIBRA, HBeAg-binding protein 3, WW, C2 and coiled-coil domain containing 1, kidney and brain protein, protein WWC1, protein phosphatase 1, regulatory subunit 168
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KIBRA/WWC1 using anti-KIBRA/WWC1 antibody (A02837-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Mcf-7 whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIBRA/WWC1 antigen affinity purified polyclonal antibody (Catalog # A02837-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIBRA/WWC1 at approximately 130KD. The expected band size for KIBRA/WWC1 is at 130KD.
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