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Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
Immunofluorescent staining of human cell line HeLa shows localization to cytosol.

Anti-YTHDF2 Antibody

HPA054080
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetYTHDF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-YTHDF2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51441
  • Target name
    YTHDF2
  • Target description
    YTH N6-methyladenosine RNA binding protein F2
  • Target synonyms
    CAHL, DF2, HGRG8, NY-REN-2, YTH domain-containing family protein 2, 9430020E02Rik, CLL-associated antigen KW-14, YTH N(6)-methyladenosine RNA binding protein 2, YTH N6-methyladenosine RNA binding protein 2, YTH domain family, member 2, high-glucose-regulated protein 8, renal carcinoma antigen NY-REN-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5A9
  • Protein Name
    YTH domain-containing family protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of YTHDF2 using anti-YTHDF2 antibody (A02621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human Hepg2 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YTHDF2 antigen affinity purified polyclonal antibody (Catalog # A02621-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YTHDF2 at approximately 65KD. The expected band size for YTHDF2 is at 65KD.
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