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Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.

Anti-YWHAE Antibody

HPA008445
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetYWHAE
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-YWHAE
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7531
  • Target name
    YWHAE
  • Target description
    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
  • Target synonyms
    14-3-3E, HEL2, KCIP-1, MDCR, MDS, 14-3-3 protein epsilon, 14-3-3 epsilon, epididymis luminal protein 2, mitochondrial import stimulation factor L subunit, protein kinase C inhibitor protein-1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide, tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62258
  • Protein Name
    14-3-3 protein epsilon
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Western Blot Positive WB detected in: A375 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, Mouse brain tissue All lanes: YWHAE antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 27 kDa Observed band size: 30 kDa
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References
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Figure 1. Western blot analysis of YWHAE using anti-YWHAE antibody (A01687-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human Raji whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates, Lane 12: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAE antigen affinity purified polyclonal antibody (Catalog # A01687-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YWHAE at approximately 28KD. The expected band size for YWHAE is at 28KD.
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Human HeLa lysates probed with Rabbit Anti-14-3-3 epsilon Polyclonal Antibody, Unconjugated (bs-2340R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
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