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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.

Anti-ZBTB7A Antibody

HPA046387
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
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Product group Antibodies
TargetZBTB7A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZBTB7A
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51341
  • Target name
    ZBTB7A
  • Target description
    zinc finger and BTB domain containing 7A
  • Target synonyms
    factor binding IST protein 1; factor that binds to inducer of short transcripts protein 1; FBI1; FBI-1; HIV-1 1st-binding protein 1; HIV-1 inducer of short transcripts binding protein; leukemia/lymphoma-related factor; LRF; lymphoma related factor; POK erythroid myeloid ontogenic factor; pokemon; pokemon 1; POZ and Krueppel erythroid myeloid ontogenic factor; TIP21; TTF-I-interacting peptide 21; ZBTB7; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein; zinc finger and BTB domain-containing protein 7A; zinc finger protein 857A; ZNF857A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95365
  • Protein Name
    Zinc finger and BTB domain-containing protein 7A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZBTB7A using anti-ZBTB7A antibody (PB9910). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB7A antigen affinity purified polyclonal antibody (Catalog # PB9910) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB7A at approximately 72 kDa. The expected band size for ZBTB7A is at 61 kDa.
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