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ProductsBack/Pokemon/ZBTB7A Polyclonal Antibody
293T lysates probed with PokemonZBTB7A Polyclonal Antibody, Unconjugated (bs-0891R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
293T lysates probed with PokemonZBTB7A Polyclonal Antibody, Unconjugated (bs-0891R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
293T lysates probed with PokemonZBTB7A Polyclonal Antibody, Unconjugated (bs-0891R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Pokemon/ZBTB7A Polyclonal Antibody

BS-0891R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetZBTB7A
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Pokemon Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID51341
  • Target name
    ZBTB7A
  • Target description
    zinc finger and BTB domain containing 7A
  • Target synonyms
    factor binding IST protein 1; factor that binds to inducer of short transcripts protein 1; FBI1; FBI-1; HIV-1 1st-binding protein 1; HIV-1 inducer of short transcripts binding protein; leukemia/lymphoma-related factor; LRF; lymphoma related factor; POK erythroid myeloid ontogenic factor; pokemon; pokemon 1; POZ and Krueppel erythroid myeloid ontogenic factor; TIP21; TTF-I-interacting peptide 21; ZBTB7; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein; zinc finger and BTB domain-containing protein 7A; zinc finger protein 857A; ZNF857A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95365
  • Protein Name
    Zinc finger and BTB domain-containing protein 7A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZBTB7A using anti-ZBTB7A antibody (PB9910). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB7A antigen affinity purified polyclonal antibody (Catalog # PB9910) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB7A at approximately 72 kDa. The expected band size for ZBTB7A is at 61 kDa.
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