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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Anti-ZC3H7A Antibody

HPA040808
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZC3H7A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZC3H7A
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29066
  • Target name
    ZC3H7A
  • Target description
    zinc finger CCCH-type containing 7A
  • Target synonyms
    HSPC055, ZC3H7, ZC3HDC7, zinc finger CCCH domain-containing protein 7A, zinc finger CCCH-type domain containing 7, zinc-finger protein AY163807
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IWR0
  • Protein Name
    Zinc finger CCCH domain-containing protein 7A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ZC3H7A using anti-ZC3H7A antibody (A15075-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZC3H7A antigen affinity purified polyclonal antibody (Catalog # A15075-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZC3H7A at approximately 111 kDa. The expected band size for ZC3H7A is at 111 kDa.
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