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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.

Anti-ZIC1 Antibody

HPA004098
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetZIC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZIC1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7545
  • Target name
    ZIC1
  • Target description
    Zic family member 1
  • Target synonyms
    BAIDCS; CRS6; ZIC; Zic family member 1 (odd-paired homolog, Drosophila); zinc finger protein 201; Zinc finger protein of the cerebellum 1; zinc finger protein ZIC 1; ZNF201
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15915
  • Protein Name
    Zinc finger protein ZIC 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZIC1 using anti-ZIC1 antibody (A05537-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC1 antigen affinity purified polyclonal antibody (Catalog # A05537-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIC1 at approximately 48 kDa. The expected band size for ZIC1 is at 48 kDa.
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