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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer.

Anti-ZIC3 Antibody

HPA052936
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZIC3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZIC3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7547
  • Target name
    ZIC3
  • Target description
    Zic family zinc finger 3
  • Target synonyms
    HTX, HTX1, VACTERLX, ZNF203, zinc finger protein ZIC 3, Zic family member 3 (odd-paired homolog, Drosophila), heterotaxy 1, zinc finger protein 203, zinc finger protein of the cerebellum 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60481
  • Protein Name
    Zinc finger protein ZIC 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ZIC3 using anti-ZIC3 antibody (A04050-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC3 antigen affinity purified polyclonal antibody (Catalog # A04050-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIC3 at approximately 56-60KD. The expected band size for ZIC3 is at 56-60KD.
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