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ZIC3 Antibody

LS-C411010
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetZIC3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ZIC3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 50kDa, while the observed MW by Western blot was 51kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 50kDa, while the observed MW by Western blot was 51kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7547
  • Target name
    ZIC3
  • Target description
    Zic family zinc finger 3
  • Target synonyms
    HTX, HTX1, VACTERLX, ZNF203, zinc finger protein ZIC 3, Zic family member 3 (odd-paired homolog, Drosophila), heterotaxy 1, zinc finger protein 203, zinc finger protein of the cerebellum 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZIC3 using anti-ZIC3 antibody (A04050-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC3 antigen affinity purified polyclonal antibody (Catalog # A04050-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIC3 at approximately 56-60KD. The expected band size for ZIC3 is at 56-60KD.
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