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Immunohistochemical staining of human ovary shows moderate cytoplasmic/ membranous positivity in oocytes.
Immunohistochemical staining of human ovary shows moderate cytoplasmic/ membranous positivity in oocytes.
Immunohistochemical staining of human ovary shows moderate cytoplasmic/ membranous positivity in oocytes.

Anti-ZP2 Antibody

HPA011296
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZP2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7783
  • Target name
    ZP2
  • Target description
    zona pellucida glycoprotein 2
  • Target synonyms
    OOMD6, OZEMA6, ZPA, Zp-2, zona pellucida sperm-binding protein 2, zona pellucida 2, zona pellucida glycoprotein 2 (sperm receptor), zona pellucida glycoprotein ZP2, zona pellucida protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05996
  • Protein Name
    Zona pellucida sperm-binding protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ZP2 using anti-ZP2 antibody (PB9912). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZP2 antigen affinity purified polyclonal antibody (Catalog # PB9912) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZP2 at approximately 82 kDa. The expected band size for ZP2 is at 82 kDa.
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