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Western blot All lanes: ZP2 antibody at 3.06ug/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 83, 82 kDa Observed band size: 83 kDa
Western blot All lanes: ZP2 antibody at 3.06ug/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 83, 82 kDa Observed band size: 83 kDa
Western blot All lanes: ZP2 antibody at 3.06ug/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 83, 82 kDa Observed band size: 83 kDa

ZP2 Antibody

CSB-PA027120ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ZP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7783
  • Target name
    ZP2
  • Target description
    zona pellucida glycoprotein 2
  • Target synonyms
    OOMD6, OZEMA6, ZPA, Zp-2, zona pellucida sperm-binding protein 2, zona pellucida 2, zona pellucida glycoprotein 2 (sperm receptor), zona pellucida glycoprotein ZP2, zona pellucida protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05996
  • Protein Name
    Zona pellucida sperm-binding protein 2
  • Scientific Description
    The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ZP2 using anti-ZP2 antibody (PB9912). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZP2 antigen affinity purified polyclonal antibody (Catalog # PB9912) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZP2 at approximately 82 kDa. The expected band size for ZP2 is at 82 kDa.
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