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ProductsBack/ANXA2 Antibody - N-terminal region (OAAB17056)

ANXA2 Antibody - N-terminal region (OAAB17056)

OAAB17056
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetANXA2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    ANXA2 Antibody - N-terminal region (OAAB17056)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Gene ID302
  • Target name
    ANXA2
  • Target description
    annexin A2
  • Target synonyms
    annexin A2; annexin II; annexin-2; ANX2; ANX2L4; CAL1H; calpactin I heavy chain; calpactin I heavy polypeptide; calpactin-1 heavy chain; chromobindin 8; epididymis secretory protein Li 270; epididymis secretory sperm binding protein; HEL-S-270; LIP2; lipocortin II; LPC2; LPC2D; P36; PAP-IV; placental anticoagulant protein IV; protein I
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Annexin A2/ANXA2 using anti-Annexin A2/ANXA2 antibody (A00868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A2/ANXA2 antigen affinity purified polyclonal antibody (Catalog # A00868-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A2/ANXA2 at approximately 36 kDa. The expected band size for Annexin A2/ANXA2 is at 39 kDa.
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