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ProductsBack/APC Anti-Human CD41 (HIP8)

APC Anti-Human CD41 (HIP8)

APC-30131
Wuhan Fine Biotech
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetITGA2B
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Overview

  • Supplier
    Wuhan Fine Biotech
  • Product Name
    APC Anti-Human CD41 (HIP8)
  • Delivery Days Customer
    999
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HIP8
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID3674
  • Target name
    ITGA2B
  • Target description
    integrin subunit alpha 2b
  • Target synonyms
    BDPLT16, BDPLT2, CD41, CD41B, FMAIT2, GP2B, GPIIb, GT, GT1, GTA, HPA3, PPP1R93, integrin alpha-IIb, GPalpha IIb, alphaIIb protein, integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41), platelet fibrinogen receptor, alpha subunit, platelet glycoprotein IIb of IIb/IIIa complex, platelet membrane glycoprotein IIb, platelet-specific antigen BAK, protein phosphatase 1, regulatory subunit 93
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08514
  • Protein Name
    Integrin alpha-IIb
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD41/Integrin Alpha 2B/ITGA2B using anti-CD41/Integrin Alpha 2B/ITGA2B antibody (A01102-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD41/Integrin Alpha 2B/ITGA2B antigen affinity purified polyclonal antibody (Catalog # A01102-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD41/Integrin Alpha 2B/ITGA2B at approximately 125 kDa. The expected band size for CD41/Integrin Alpha 2B/ITGA2B is at 113 kDa.
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Lane 1: Human HL60 cell lysates; Lane 2: Human Raji cell lysates probed with CD41/Integrin alpha 2b Monoclonal Antibody, Unconjugated (bsm-33112M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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