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ProductsBack/APCS / Serum Amyloid P / SAP Antibody

APCS / Serum Amyloid P / SAP Antibody

LS-C663198
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetAPCS
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    APCS / Serum Amyloid P / SAP Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID325
  • Target name
    APCS
  • Target description
    amyloid P component, serum
  • Target synonyms
    9.5S alpha-1-glycoprotein; epididymis secretory sperm binding protein Li 92n; HEL-S-92n; pentaxin-related; pentraxin-2; pentraxin-related; PTX2; SAP; serum amyloid P-component
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Serum Amyloid P/APCS using anti-Serum Amyloid P/APCS antibody (A00162). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P/APCS antigen affinity purified polyclonal antibody (Catalog # A00162) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Serum Amyloid P/APCS at approximately 25-29 kDa. The expected band size for Serum Amyloid P/APCS is at 25 kDa.
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