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ProductsBack/Apg3 (ATG3) Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ATG3 antibody.
Western blot analysis of extracts of various cell lines, using ATG3 antibody.
Western blot analysis of extracts of various cell lines, using ATG3 antibody.

Apg3 (ATG3) Rabbit Polyclonal Antibody

TA332928
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATG3
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Apg3 (ATG3) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID64422
  • Target name
    ATG3
  • Target description
    autophagy related 3
  • Target synonyms
    APG3, APG3-LIKE, APG3L, PC3-96, hApg3, ubiquitin-like-conjugating enzyme ATG3, APG3 autophagy 3-like, ATG3 autophagy related 3 homolog, autophagy-related protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NT62
  • Protein Name
    Ubiquitin-like-conjugating enzyme ATG3
  • Scientific Description
    Rabbit anti-ATG3 Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Apg3 using anti-Apg3 antibody (A01768-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apg3 antigen affinity purified polyclonal antibody (Catalog # A01768-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apg3 at approximately 36-40 kDa. The expected band size for Apg3 is at 36 kDa.
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