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APOBEC3B Antibody

CAB9010
Assay Genie
TargetAPOBEC3B
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    APOBEC3B Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID9582
  • Target name
    APOBEC3B
  • Target description
    apolipoprotein B mRNA editing enzyme catalytic subunit 3B
  • Target synonyms
    A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2, DNA dC->dU-editing enzyme APOBEC-3B, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B, cytidine deaminase, phorbolin 2, phorbolin 3, phorbolin-1-related protein, phorbolin-2/3, probable DNA dC->dU-editing enzyme APOBEC-3B
  • Host
    Rabbit
  • Protein IDQ9UH17
  • Protein Name
    DNA dC->dU-editing enzyme APOBEC-3B
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APOBEC3B using anti-APOBEC3B antibody (A01088-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3B antigen affinity purified polyclonal antibody (Catalog # A01088-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3B at approximately 35 kDa. The expected band size for APOBEC3B is at 46 kDa.
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The data was published in the journal Biochem Biophys Rep in 2018. PMID: 30417129
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References
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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
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