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ProductsBack/APOBEC3G Antibody - middle region (Phospho-E133)

APOBEC3G Antibody - middle region (Phospho-E133)

OAAB09815
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAPOBEC3G
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    APOBEC3G Antibody - middle region (Phospho-E133)
  • Delivery Days Customer
    23
  • Application Supplier Note
    WB: 1:1000 IHC-P: 1:50~100
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Gene ID60489
  • Target name
    APOBEC3G
  • Target description
    apolipoprotein B mRNA editing enzyme catalytic subunit 3G
  • Target synonyms
    A3G, ARCD, ARP-9, ARP9, CEM-15, CEM15, MDS019, bK150C2.7, dJ494G10.1, DNA dC->dU-editing enzyme APOBEC-3G, APOBEC-related cytidine deaminase, APOBEC-related protein 9, DNA dC->dU editing enzyme, apolipoprotein B editing enzyme catalytic polypeptide-like 3G, apolipoprotein B mRNA editing enzyme cytidine deaminase, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G, apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3G, deoxycytidine deaminase, phorbolin-like protein MDS019
  • Host
    Rabbit
  • Protein IDQ9HC16
  • Protein Name
    DNA dC->dU-editing enzyme APOBEC-3G
  • Scientific Description
    Purified Rabbit Polyclonal Antibody (Pab)
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APOBEC3G using anti-APOBEC3G antibody (PB9985). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3G antigen affinity purified polyclonal antibody (Catalog # PB9985) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3G at approximately 46 kDa. The expected band size for APOBEC3G is at 46 kDa.
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