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ProductsBack/Apolipoprotein A I (APOA1) (25-267) Mouse Monoclonal Antibody [Clone ID: AT1E12]
Western blot analysis: The extracts of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APOA1 (1/500 ~ 1/5,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis: The extracts of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APOA1 (1/500 ~ 1/5,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis: The extracts of HepG2 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APOA1 (1/500 ~ 1/5,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Apolipoprotein A I (APOA1) (25-267) Mouse Monoclonal Antibody [Clone ID: AT1E12]

AM39003PU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetAPOA1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Apolipoprotein A I (APOA1) (25-267) Mouse Monoclonal Antibody [Clone ID: AT1E12]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT1E12
  • Gene ID335
  • Target name
    APOA1
  • Target description
    apolipoprotein A1
  • Target synonyms
    AMYLD3, HPALP2, apo(a), apolipoprotein A-I, apo-AI, epididymis secretory sperm binding protein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP02647
  • Protein Name
    Apolipoprotein A-I
  • Scientific Description
    Apolipoprotein A I (APOA1) (25-267) mouse monoclonal antibody, clone AT1E12, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of APOA1 using anti-APOA1 antibody (PB9916). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOA1 antigen affinity purified polyclonal antibody (Catalog # PB9916) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOA1 at approximately 26 kDa. The expected band size for APOA1 is at 31 kDa.
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