Mouse plasma and Rat plasma (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody [HL1713] (GTX637312) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody [HL1713] (GTX637312) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637312/GTX637312_44823_20221007_WB_plasma_22101319_173.webp "Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody [HL1713] (GTX637312) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Apolipoprotein A1 antibody [HL1713] detects Apolipoprotein A1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody [HL1713] (GTX637312) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637312/GTX637312_T-44774_20220916_ICC_IF_22102723_381.webp "Apolipoprotein A1 antibody [HL1713] detects Apolipoprotein A1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody [HL1713] (GTX637312) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, full-length human Apo-A1 Protein, His Tag (134.23-2.1 nM). Coated protein was probed with Apolipoprotein A1 antibody [HL1713] (GTX637312) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637312/GTX637312_T-44774_20221102_ELISA_Indirect_22110919_200.webp "Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, full-length human Apo-A1 Protein, His Tag (134.23-2.1 nM). Coated protein was probed with Apolipoprotein A1 antibody [HL1713] (GTX637312) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
Mouse plasma and Rat plasma (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody [HL1713] (GTX637312) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Apolipoprotein A1 antibody [HL1713]
GTX637312
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAPOA1
Overview
- SupplierGeneTex
- Product NameApolipoprotein A1 antibody [HL1713]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1713
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID335
- Target nameAPOA1
- Target descriptionapolipoprotein A1
- Target synonymsAMYLD3, HPALP2, apo(a), apolipoprotein A-I, apo-AI, epididymis secretory sperm binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP02647
- Protein NameApolipoprotein A-I
- Scientific DescriptionThis gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![WB analysis of HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY APOA1 cDNA (2) using GTX83043 Apolipoprotein A1 antibody [5F4F5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83043/GTX83043_20170912_WB_w_23061322_497.webp)
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