IHC-P analysis of human liver tissue using GTX15664 Apolipoprotein B antibody [F2C4]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20
![ICC/IF analysis of HepG2 cells using GTX15664 Apolipoprotein B antibody [F2C4]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15664/GTX15664_277_ICC-IF_w_23060620_433.webp "ICC/IF analysis of HepG2 cells using GTX15664 Apolipoprotein B antibody [F2C4]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature")
IHC-P analysis of human liver tissue using GTX15664 Apolipoprotein B antibody [F2C4]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20
Apolipoprotein B antibody [F2C4]
GTX15664
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
Product group Antibodies
ReactivityHuman
TargetAPOB
Overview
- SupplierGeneTex
- Product NameApolipoprotein B antibody [F2C4]
- Delivery Days Customer9
- Application Supplier NoteIHC-P: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDF2C4
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID338
- Target nameAPOB
- Target descriptionapolipoprotein B
- Target synonymsapoB-100; apoB-48; apolipoprotein B (including Ag(x) antigen); apolipoprotein B-100; apolipoprotein B48; FCHL2; FLDB; LDLCQ4
- HostMouse
- IsotypeIgG2a
- Protein IDP04114
- Protein NameApolipoprotein B-100
- Scientific DescriptionThis gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203