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APPL Antibody (YA2135, Rabbit)

HY-P82390
MedChem Express
TargetAPPL1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    APPL Antibody (YA2135, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID26060
  • Target name
    APPL1
  • Target description
    adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
  • Target synonyms
    adapter protein containing PH domain, PTB domain and leucine zipper motif 1; adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1; AKT2 interactor; APPL; DCC-interacting protein 13-alpha; DIP13alpha; dip13-alpha; MODY14; signaling adaptor protein DIP13alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    APPL Antibody (YA2135) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2135), targeting APPL, with a predicted molecular weight of 80 kDa (observed band size: 80 kDa). APPL Antibody (YA2135) can be used for WB, IHC-P experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APPL1 using anti-APPL1 antibody (A02381). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APPL1 antigen affinity purified polyclonal antibody (Catalog # A02381) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APPL1 at approximately 85 kDa. The expected band size for APPL1 is at 80 kDa.
Product group Antibodies
References
Boster Bio
Anti-APPL/APPL1 Antibody Picoband(r)A02381-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetAPPL1
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Product group Antibodies
Everest Biotech
Goat anti-APPL / DIP13alphaEB06075
ApplicationsWestern Blot, ELISA
TargetAPPL1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-APPL1/DIP13alpha Antibody, Affinity PurifiedA304-782A
ApplicationsImmunoPrecipitation, Western Blot
TargetAPPL1
  • SizePrice
Product group Antibodies
Assay Genie
APPL1 AntibodyCAB4606
TargetAPPL1
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Biomatik
Adaptor Protein Phosphotyrosine Interaction PH Domain And leucine Zipper Containing Protein 1 (APPL1) Polyclonal AntibodyCAU21504
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetAPPL1
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Product group Antibodies
Bioss Antibodies
APPL1 Recombinant AntibodyBSM-61064R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetAPPL1
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Abbexa
APPL AntibodyABX430193
ApplicationsWestern Blot, ELISA
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Antibodies.com
Anti-APPL AntibodyA83958
ApplicationsWestern Blot, ELISA
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Product group Antibodies
RayBiotech
Anti-APPL1 Antibody144-04606
ApplicationsWestern Blot
TargetAPPL1
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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
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Atlas Antibodies
Anti-APPL1 AntibodyHPA011138
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TargetAPPL1
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