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Western Blot analysis of A549 cells using APPL1 Polyclonal Antibody
Western Blot analysis of A549 cells using APPL1 Polyclonal Antibody
Western Blot analysis of A549 cells using APPL1 Polyclonal Antibody

APPL1 Antibody

CSB-PA000908
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetAPPL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    APPL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26060
  • Target name
    APPL1
  • Target description
    adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
  • Target synonyms
    APPL, DIP13alpha, MODY14, DCC-interacting protein 13-alpha, AKT2 interactor, adapter protein containing PH domain, PTB domain and leucine zipper motif 1, adaptor protein containing pH domain, PTB domain and leucine zipper motif 1, adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1, dip13-alpha, signaling adaptor protein DIP13alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKG1
  • Protein Name
    DCC-interacting protein 13-alpha
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APPL1 using anti-APPL1 antibody (A02381). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APPL1 antigen affinity purified polyclonal antibody (Catalog # A02381) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APPL1 at approximately 85 kDa. The expected band size for APPL1 is at 80 kDa.
Product group Antibodies
References
Boster Bio
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TargetAPPL1
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RayBiotech
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Biomatik
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