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AQP1 Antibody (OAAB19511)

OAAB19511
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat, Sheep
TargetAQP1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    AQP1 Antibody (OAAB19511)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Format
    Liquid; 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
  • Gene ID358
  • Target name
    AQP1
  • Target description
    aquaporin 1 (Colton blood group)
  • Target synonyms
    AQP-CHIP, CHIP28, CO, aquaporin-1, Colton blood group antigen, aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group), aquaporin 1, Colton blood group antigen, aquaporin-CHIP, bloodgroup CO protein, channel-like integral membrane protein of 28 kDa, channel-like integral membrane protein, 28-kDa, urine water channel, water channel protein for red blood cells and kidney proximal tubule
  • Host
    Rabbit
  • Reactivity
    Canine, Human, Mouse, Rat, Sheep
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aquaporin 1/AQP1 using anti-Aquaporin 1/AQP1 antibody (A00865). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 1/AQP1 antigen affinity purified polyclonal antibody (Catalog # A00865) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 1/AQP1 at approximately 23 kDa. The expected band size for Aquaporin 1/AQP1 is at 23 kDa.
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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Aquaporin 1 antibody (GTX132870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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