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AQP2 Polyclonal Antibody

RD76182A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAQP2
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    AQP2 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.7 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID359
  • Target name
    AQP2
  • Target description
    aquaporin 2
  • Target synonyms
    AQP-CD, NDI2, WCH-CD, aquaporin-2, ADH water channel, AQP-2, aquaporin 2 (collecting duct), aquaporin-CD, collecting duct water channel protein, water channel protein for renal collecting duct, water-channel aquaporin 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    The basic job of aquaporin 2 is to reabsorb water from the urine while its being removed from the blood by the kidney. Aquaporin 2 is in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes, but when it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that causes the aquaporin 2 containing vesicles to fuse with the plasma membrane so the aquaporin 2 can be used by the cell.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of Aquaporin 2 using anti-Aquaporin 2 antibody (PB9474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat NRK whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse HBZY whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 2 antigen affinity purified polyclonal antibody (Catalog # PB9474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 2 at approximately 26 kDa. The expected band size for Aquaporin 2 is at 26 kDa.
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