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Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using AQP5 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using AQP5 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using AQP5 Polyclonal Antibody at dilution 1:40

AQP5 Polyclonal Antibody

E-AB-15511
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    AQP5 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    Aquaporin-5is aproteinthat in humans is encoded by theAQP5gene. Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aquaporin 5 using anti-Aquaporin 5 antibody (A03085). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 5 antigen affinity purified polyclonal antibody (Catalog # A03085) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 5 at approximately 28KD. The expected band size for Aquaporin 5 is at 28KD.
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