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Aquaporin 1 antibody [1/A5F6]

GTX11023
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAQP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Aquaporin 1 antibody [1/A5F6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/1,000-1/5,000. IHC-P: 1/500-1/1,000. IHC-Fr: 1/500-1/1,000. ELISA: 1/1000-1/20,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1/A5F6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID358
  • Target name
    AQP1
  • Target description
    aquaporin 1 (Colton blood group)
  • Target synonyms
    AQP-CHIP; aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group); aquaporin 1, Colton blood group antigen; aquaporin-1; aquaporin-CHIP; channel-like integral membrane protein, 28-kDa; CHIP28; CO; Colton blood group antigen; urine water channel; water channel protein for red blood cells and kidney proximal tubule
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP29972
  • Protein Name
    Aquaporin-1
  • Scientific Description
    This gene encodes a small integral membrane protein with six bilayer spanning domains that functions as a water channel protein. This protein permits passive transport of water along an osmotic gradient. This gene is a possible candidate for disorders involving imbalance in ocular fluid movement. [provided by RefSeq, Aug 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Databases for technical aspects of immunohistochemistry: 2021 update. Moroki T et al., 2021 Apr, J Toxicol Pathol
    Read more
  • Expression of beta-catenin in regenerating renal tubules of cisplatin-induced kidney failure in rats. Terada N et al., 2018 Dec, Clin Exp Nephrol
    Read more
  • Immunolocalization of beta-catenin, E-cadherin and N-cadherin in neonate and adult rat kidney. Terada N et al., 2017 Nov 10, J Vet Med Sci
    Read more
  • Characterization of pancreatic islet cell tumors and renal tumors induced by a combined treatment of streptozotocin and nicotinamide in male SD rats. Kato Y et al., 2017 Sep 5, Exp Toxicol Pathol
    Read more
  • Simultaneous changes of magnetic resonance diffusion-weighted imaging and pathological microstructure in locally advanced cervical cancer caused by neoadjuvant chemotherapy. Fu C et al., 2015 Aug, J Magn Reson Imaging
    Read more
  • Does Aquaporin-1 expression have clinical significance in serous epithelial ovarian cancer? Takal MK et al., 2013, J Turk Ger Gynecol Assoc
    Read more

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Figure 1. Western blot analysis of Aquaporin 1/AQP1 using anti-Aquaporin 1/AQP1 antibody (A00865). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 1/AQP1 antigen affinity purified polyclonal antibody (Catalog # A00865) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 1/AQP1 at approximately 23 kDa. The expected band size for Aquaporin 1/AQP1 is at 23 kDa.
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