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ARA70 / NCOA4 Antibody

LS-C334222
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNCOA4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ARA70 / NCOA4 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 32kDa/69kDa/71kDa/73kDa, while the observed MW by Western blot was 70kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 32kDa/69kDa/71kDa/73kDa, while the observed MW by Western blot was 70kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8031
  • Target name
    NCOA4
  • Target description
    nuclear receptor coactivator 4
  • Target synonyms
    ARA70, ELE1, PTC3, RFG, nuclear receptor coactivator 4, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, NCoA-4, RET-activating gene ELE1, androgen receptor-associated protein of 70 kDa, ferritin cargo receptor NCOA4, ret fused
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCOA4 using anti-NCOA4 antibody (A04368-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOA4 antigen affinity purified polyclonal antibody (Catalog # A04368-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCOA4 at approximately 70 kDa. The expected band size for NCOA4 is at 70 kDa.
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