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Western Blot analysis of HeLa cells using A-Raf Polyclonal Antibody
Western Blot analysis of HeLa cells using A-Raf Polyclonal Antibody
Western Blot analysis of HeLa cells using A-Raf Polyclonal Antibody

ARAF Antibody

CSB-PA000918
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetARAF
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Overview

  • Supplier
    Cusabio
  • Product Name
    ARAF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID369
  • Target name
    ARAF
  • Target description
    A-Raf proto-oncogene, serine/threonine kinase
  • Target synonyms
    A-RAF; A-Raf proto-oncogene serine/threonine-protein kinase; ARAF1; Oncogene ARAF1; PKS2; proto-oncogene A-Raf-1; proto-oncogene Pks; RAFA1; Ras-binding protein DA-Raf; serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10398
  • Protein Name
    Serine/threonine-protein kinase A-Raf
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARAF using anti-ARAF antibody (A02061-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARAF antigen affinity purified polyclonal antibody (Catalog # A02061-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARAF at approximately 68 kDa. The expected band size for ARAF is at 68 kDa.
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