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ICC/IF analysis of PFA-fixed HeLa cells using GTX04096 ARC antibody. Red : Primary antibody Green : beta tubulin Blue : DAPI Permeabilize : 0.1% Triton X-100 Dilution : 1:200
ICC/IF analysis of PFA-fixed HeLa cells using GTX04096 ARC antibody. Red : Primary antibody Green : beta tubulin Blue : DAPI Permeabilize : 0.1% Triton X-100 Dilution : 1:200
ICC/IF analysis of PFA-fixed HeLa cells using GTX04096 ARC antibody. Red : Primary antibody Green : beta tubulin Blue : DAPI Permeabilize : 0.1% Triton X-100 Dilution : 1:200

ARC antibody

GTX04096
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetARC
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Overview

  • Supplier
    GeneTex
  • Product Name
    ARC antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:100-1:500. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23237
  • Target name
    ARC
  • Target description
    activity regulated cytoskeleton associated protein
  • Target synonyms
    activity-regulated cytoskeleton-associated protein; activity-regulated gene 3.1 protein homolog; ARC/ARG3.1; Arg3.1; hArc
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7LC44
  • Protein Name
    Activity-regulated cytoskeleton-associated protein
  • Scientific Description
    Predicted to enable mRNA binding activity. Involved in cell migration; cytoskeleton organization; and regulation of cell morphogenesis. Located in cytoplasm and plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Arc using anti-Arc antibody (PB9753). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arc antigen affinity purified polyclonal antibody (Catalog # PB9753) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arc at approximately 45 kDa. The expected band size for Arc is at 45 kDa.
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