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Anti-AREG Antibody

CAB12680
Assay Genie
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetAREG
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-AREG Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID374
  • Target name
    AREG
  • Target description
    amphiregulin
  • Target synonyms
    AR, AREGB, CRDGF, SDGF, amphiregulin, amphiregulin B, colorectum cell-derived growth factor, schwannoma-derived growth factor
  • Host
    Rabbit
  • Protein IDP15514
  • Protein Name
    Amphiregulin
  • Scientific Description
    AREG Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for IF/ICC, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Amphiregulin/AREG using anti-Amphiregulin/AREG antibody (A01787-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin/AREG antigen affinity purified polyclonal antibody (Catalog # A01787-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin/AREG at approximately 45 kDa. The expected band size for Amphiregulin/AREG is at 28 kDa.
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