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Western blot All lanes: Arginase-1 antibody at 8ug/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 36, 26 kDa Observed band size: 35 kDa
Western blot All lanes: Arginase-1 antibody at 8ug/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 36, 26 kDa Observed band size: 35 kDa
Western blot All lanes: Arginase-1 antibody at 8ug/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 36, 26 kDa Observed band size: 35 kDa

ARG1 Antibody

CSB-PA002005ESR1HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetARG1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ARG1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID383
  • Target name
    ARG1
  • Target description
    arginase 1
  • Target synonyms
    arginase-1, arginase, liver, liver-type arginase, type I arginase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05089
  • Protein Name
    Arginase-1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ARG1 using anti-ARG1 antibody (A01106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: monkey liver tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG1 antigen affinity purified polyclonal antibody (Catalog # A01106) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARG1 at approximately 37 kDa. The expected band size for ARG1 is at 35 kDa.
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Western Blot Positive WB detected in: Mouse liver tissue, Rat liver tissue All lanes: ARG1 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 36, 26 kDa Observed band size: 35 kDa
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