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Western Blot analysis of K562 cells using Arginase II Polyclonal Antibody
Western Blot analysis of K562 cells using Arginase II Polyclonal Antibody
Western Blot analysis of K562 cells using Arginase II Polyclonal Antibody

ARG2 Antibody

CSB-PA000920
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetARG2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ARG2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID384
  • Target name
    ARG2
  • Target description
    arginase 2
  • Target synonyms
    arginase II; arginase, type II; arginase-2, mitochondrial; kidney arginase; kidney-type arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; nonhepatic arginase; non-hepatic arginase; type II arginase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78540
  • Protein Name
    Arginase-2, mitochondrial
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARG2 using anti-ARG2 antibody (A02244-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Sw620 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG2 antigen affinity purified polyclonal antibody (Catalog # A02244-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARG2 at approximately 39KD. The expected band size for ARG2 is at 39KD.
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