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DU145 Cell lysates; probed with Arginase II Polyclonal Antibody, unconjugated (bs-11397R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
DU145 Cell lysates; probed with Arginase II Polyclonal Antibody, unconjugated (bs-11397R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
DU145 Cell lysates; probed with Arginase II Polyclonal Antibody, unconjugated (bs-11397R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Arginase II Polyclonal Antibody

BS-11397R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetARG2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Arginase 2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID384
  • Target name
    ARG2
  • Target description
    arginase 2
  • Target synonyms
    arginase II; arginase, type II; arginase-2, mitochondrial; kidney arginase; kidney-type arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; nonhepatic arginase; non-hepatic arginase; type II arginase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78540
  • Protein Name
    Arginase-2, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Isolation and characterization of ovine monocyte-derived macrophages from peripheral blood. Liu Y et al., 2018 Nov, Vet Immunol Immunopathol
    Read more
  • Critical role for arginase 2 in obesity-associated pancreatic cancer. Zaytouni T et al., 2017 Aug 14, Nat Commun
    Read more

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Figure 1. Western blot analysis of ARG2 using anti-ARG2 antibody (A02244-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Sw620 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG2 antigen affinity purified polyclonal antibody (Catalog # A02244-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARG2 at approximately 39KD. The expected band size for ARG2 is at 39KD.
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