ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60370 Argonautes antibody [GT9003]. Red : Primary antibody Blue : DAPI Dilution : 1:500
![IP analysis of HeLa cells (UV crosslinked and lysaed, and the the lysate was treated with DNase I) using GTX60370 Argonautes antibody [GT9003]. 10 microl of input (10% of starting material), 10 microl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded. Antibody amount : 4 or 12microg.](https://www.genetex.com/upload/website/prouct_img/normal/GTX60370/GTX60370_20201117_IP_156_w_23061123_728.webp "IP analysis of HeLa cells (UV crosslinked and lysaed, and the the lysate was treated with DNase I) using GTX60370 Argonautes antibody [GT9003]. 10 microl of input (10% of starting material), 10 microl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded. Antibody amount : 4 or 12microg.")
![RIP (RNA immunopreciptation) analysis of HeLa cells overexpressing cell lysate using GTX60370 Argonautes antibody [GT9003]. HeLa cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target A allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target G allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele).](https://www.genetex.com/upload/website/prouct_img/normal/GTX60370/GTX60370_20201117_RIP_157_w_23061123_461.webp "RIP (RNA immunopreciptation) analysis of HeLa cells overexpressing cell lysate using GTX60370 Argonautes antibody [GT9003]. HeLa cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target A allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target G allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele).")
![WB analysis of whole cell extracts (40 microg) from HeLa cells using GTX60370 Argonautes antibody [GT9003]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX60370/GTX60370_20201117_WB_158_w_23061123_767.webp "WB analysis of whole cell extracts (40 microg) from HeLa cells using GTX60370 Argonautes antibody [GT9003]. Dilution : 1:500")
ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60370 Argonautes antibody [GT9003]. Red : Primary antibody Blue : DAPI Dilution : 1:500
Argonautes antibody [GT9003]
GTX60370
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
Product group Antibodies
ReactivityHuman, Mouse
TargetAGO2
Overview
- SupplierGeneTex
- Product NameArgonautes antibody [GT9003]
- Delivery Days Customer9
- Antibody SpecificityThe antibody also recognizes other Ago family members although with the highest affinity for Ago2.
- Application Supplier NoteWB: 1:500. ICC/IF: 1:500. IP: 8 microg per IP. IHC: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT9003
- Concentration2.4 mg/ml
- ConjugateUnconjugated
- Gene ID27161
- Target nameAGO2
- Target descriptionargonaute RISC catalytic component 2
- Target synonymsargonaute 2, RISC catalytic component; cancer susceptibility candidate 7 (non-protein coding); CASC7; EIF2C2; eukaryotic translation initiation factor 2C, 2; LESKRES; LINC00980; long intergenic non-protein coding RNA 980; PAZ Piwi domain protein; PPD; protein argonaute-2; protein slicer; Q10
- HostMouse
- IsotypeIgG1
- Protein IDQ9UKV8
- Protein NameProtein argonaute-2
- Scientific DescriptionThis gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Blockade of miR-3614 maturation by IGF2BP3 increases TRIM25 expression and promotes breast cancer cell proliferation. Wang Z et al., 2019 Mar, EBioMedicineRead more