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Western Blot analysis of Jurkat cells using RhoGEF p115 Polyclonal Antibody
Western Blot analysis of Jurkat cells using RhoGEF p115 Polyclonal Antibody
Western Blot analysis of Jurkat cells using RhoGEF p115 Polyclonal Antibody

ARHGEF1 Antibody

CSB-PA003977
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetARHGEF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ARHGEF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9138
  • Target name
    ARHGEF1
  • Target description
    Rho guanine nucleotide exchange factor 1
  • Target synonyms
    115 kDa guanine nucleotide exchange factor; 115-kD protein; GEF1; IMD62; LBCL2; LSC; Lsc homolog; p115RhoGEF; P115-RHOGEF; Rho guanine nucleotide exchange factor (GEF) 1; rho guanine nucleotide exchange factor 1; SUB1.5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92888
  • Protein Name
    Rho guanine nucleotide exchange factor 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ARHGEF1 using anti-ARHGEF1 antibody (PB10045). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: HELA whole cell lysates, Lane 3: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF1 antigen affinity purified polyclonal antibody (Catalog # PB10045) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF1 at approximately 120 kDa. The expected band size for ARHGEF1 is at 102 kDa.
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