More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ARHGEF1 antibody
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35236 ARHGEF1 antibody. Dilution : 1:500 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35236 ARHGEF1 antibody. Dilution : 1:500 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35236 ARHGEF1 antibody. Dilution : 1:500 Loading : 25microg per lane

ARHGEF1 antibody

GTX35236
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetARHGEF1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    ARHGEF1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9138
  • Target name
    ARHGEF1
  • Target description
    Rho guanine nucleotide exchange factor 1
  • Target synonyms
    GEF1, IMD62, LBCL2, LSC, P115-RHOGEF, SUB1.5, rho guanine nucleotide exchange factor 1, 115 kDa guanine nucleotide exchange factor, 115-kD protein, Lsc homolog, Rho guanine nucleotide exchange factor (GEF) 1, p115RhoGEF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92888
  • Protein Name
    Rho guanine nucleotide exchange factor 1
  • Scientific Description
    Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of Jurkat cells using RhoGEF p115 Polyclonal Antibody
Product group Antibodies
Cusabio
ARHGEF1 AntibodyCSB-PA003977
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetARHGEF1
  • SizePrice
WB analysis of HUVEC (A), Raw264.7 (B) whole cell lysates using GTX55910 ARHGEF1 antibody.
Product group Antibodies
GeneTex
ARHGEF1 antibodyGTX55910
ApplicationsWestern Blot
TargetARHGEF1
  • SizePrice
Anti-ARHGEF1 Antibody
Product group Antibodies
Antibodies.com
Anti-ARHGEF1 AntibodyA96598
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-p115-RhoGEF Antibody, Affinity PurifiedA302-701A
ApplicationsImmunoPrecipitation, Western Blot
TargetARHGEF1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ARHGEF1 Antibody144-61386
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetARHGEF1
  • SizePrice
Figure 1. Western blot analysis of ARHGEF1 using anti-ARHGEF1 antibody (PB10045). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: HELA whole cell lysates, Lane 3: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF1 antigen affinity purified polyclonal antibody (Catalog # PB10045) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF1 at approximately 120 kDa. The expected band size for ARHGEF1 is at 102 kDa.
Product group Antibodies
Boster Bio
Anti-ARHGEF1 Antibody Picoband(r)PB10045-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetARHGEF1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ARHGEF1 Polyclonal Antibody, Unconjugated (bs-55014R) at 1:100 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss
ARHGEF1 Polyclonal AntibodyBS-55014R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetARHGEF1
  • SizePrice
Product group Antibodies
Assay Genie
[KO Validated] ARHGEF1 AntibodyCAB4274
TargetARHGEF1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ARHGEF1 AntibodyLS-C401278
ApplicationsELISA, ImmunoHistoChemistry
TargetARHGEF1
  • SizePrice
Immunohistochemistry analysis in human lymph node and liver tissues using HPA012924 antibody. Corresponding ARHGEF1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-ARHGEF1 AntibodyHPA012924
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetARHGEF1
  • SizePrice