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WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35236 ARHGEF1 antibody. Dilution : 1:500 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35236 ARHGEF1 antibody. Dilution : 1:500 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35236 ARHGEF1 antibody. Dilution : 1:500 Loading : 25microg per lane

ARHGEF1 antibody

GTX35236
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetARHGEF1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ARHGEF1 antibody - KO/KD Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9138
  • Target name
    ARHGEF1
  • Target description
    Rho guanine nucleotide exchange factor 1
  • Target synonyms
    115 kDa guanine nucleotide exchange factor; 115-kD protein; GEF1; IMD62; LBCL2; LSC; Lsc homolog; p115RhoGEF; P115-RHOGEF; Rho guanine nucleotide exchange factor (GEF) 1; rho guanine nucleotide exchange factor 1; SUB1.5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92888
  • Protein Name
    Rho guanine nucleotide exchange factor 1
  • Scientific Description
    Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARHGEF1 using anti-ARHGEF1 antibody (PB10045). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: HELA whole cell lysates, Lane 3: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF1 antigen affinity purified polyclonal antibody (Catalog # PB10045) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF1 at approximately 120 kDa. The expected band size for ARHGEF1 is at 102 kDa.
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