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Immunofluorescence staining of PC3 cells with CSB-PA019681HA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of PC3 cells with CSB-PA019681HA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of PC3 cells with CSB-PA019681HA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ARHGEF18 Antibody

CSB-PA019681HA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetARHGEF18
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Overview

  • Supplier
    Cusabio
  • Product Name
    ARHGEF18 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23370
  • Target name
    ARHGEF18
  • Target description
    Rho/Rac guanine nucleotide exchange factor 18
  • Target synonyms
    114 kDa Rho-specific guanine nucleotide exchange factor; p114RhoGEF; P114-RhoGEF; rho guanine nucleotide exchange factor 18; Rho/Rac guanine nucleotide exchange factor (GEF) 18; Rho-specific guanine nucleotide exchange factor p114; RP78; SA-RhoGEF; septin-associated RhoGEF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZSZ5
  • Protein Name
    Rho guanine nucleotide exchange factor 18
  • Scientific Description
    Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGEF18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGEF18 (1:2000).
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