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Western Blot analysis of Hela and K562 cell using ARHGEF5 Polyclonal Antibody at dilution of 1:1150
Western Blot analysis of Hela and K562 cell using ARHGEF5 Polyclonal Antibody at dilution of 1:1150
Western Blot analysis of Hela and K562 cell using ARHGEF5 Polyclonal Antibody at dilution of 1:1150

ARHGEF5 Polyclonal Antibody

E-AB-10830
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ARHGEF5 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARHGEF5 using anti-ARHGEF5 antibody (A01487-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF5 antigen affinity purified polyclonal antibody (Catalog # A01487-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF5 at approximately 250 kDa. The expected band size for ARHGEF5 is at 177 kDa.
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