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ARID5A Antibody (aa110-159)

LS-C81609
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetARID5A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ARID5A Antibody (aa110-159)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10865
  • Target name
    ARID5A
  • Target description
    AT-rich interaction domain 5A
  • Target synonyms
    ARID domain-containing protein 5A; AT rich interactive domain 5A (MRF1-like); AT-rich interactive domain-containing protein 5A; modulator recognition factor 1; modulator recognition factor I; MRF1; MRF-1; RFVG5814; RP11-363D14
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARID5A using anti-ARID5A antibody (A14678-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID5A antigen affinity purified polyclonal antibody (Catalog # A14678-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARID5A at approximately 64KD. The expected band size for ARID5A is at 64KD.
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