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ProductsBack/ARID5A antibody [GT1683]
ARID5A antibody [GT1683] detects ARID5A protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse mouse cerebellum. Green: ARID5A stained by ARID5A antibody [GT1683] (GTX631939) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
ARID5A antibody [GT1683] detects ARID5A protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse mouse cerebellum. Green: ARID5A stained by ARID5A antibody [GT1683] (GTX631939) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
ARID5A antibody [GT1683] detects ARID5A protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse mouse cerebellum. Green: ARID5A stained by ARID5A antibody [GT1683] (GTX631939) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

ARID5A antibody [GT1683]

GTX631939
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetARID5A
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Overview

  • Supplier
    GeneTex
  • Product Name
    ARID5A antibody [GT1683]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FCM: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1683
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10865
  • Target name
    ARID5A
  • Target description
    AT-rich interaction domain 5A
  • Target synonyms
    MRF-1, MRF1, RP11-363D14, AT-rich interactive domain-containing protein 5A, ARID domain-containing protein 5A, AT rich interactive domain 5A (MRF1-like), RFVG5814, modulator recognition factor 1, modulator recognition factor I
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ03989
  • Protein Name
    AT-rich interactive domain-containing protein 5A
  • Scientific Description
    Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth (Patsialou et al., 2005 [PubMed 15640446]).[supplied by OMIM]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ARID5A using anti-ARID5A antibody (A14678-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID5A antigen affinity purified polyclonal antibody (Catalog # A14678-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARID5A at approximately 64KD. The expected band size for ARID5A is at 64KD.
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