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Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Aspartate Aminotransferase Polyclonal Antibody, Unconjugated (bs-3977R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Aspartate Aminotransferase Polyclonal Antibody, Unconjugated (bs-3977R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Aspartate Aminotransferase Polyclonal Antibody, Unconjugated (bs-3977R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Aspartate aminotransferase Antibody

BS-3977R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGOT1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Aspartate aminotransferase Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2805
  • Target name
    GOT1
  • Target description
    glutamic-oxaloacetic transaminase 1
  • Target synonyms
    aspartate aminotransferase 1; aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; AST1; ASTQTL1; cAspAT; cCAT; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; GIG18; glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1, soluble; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; transaminase A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17174
  • Protein Name
    Aspartate aminotransferase, cytoplasmic
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA009679HA01HU diluted at 1:250 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of Aspartate Aminotransferase/GOT1 using anti-Aspartate Aminotransferase/GOT1 antibody (A04085-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aspartate Aminotransferase/GOT1 antigen affinity purified polyclonal antibody (Catalog # A04085-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aspartate Aminotransferase/GOT1 at approximately 41KD. The expected band size for Aspartate Aminotransferase/GOT1 is at 46KD.
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