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Western Blot analysis of HeLa cells using ATF-2 Polyclonal Antibody
Western Blot analysis of HeLa cells using ATF-2 Polyclonal Antibody
Western Blot analysis of HeLa cells using ATF-2 Polyclonal Antibody

ATF2 Antibody

CSB-PA000946
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetATF2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATF2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1386
  • Target name
    ATF2
  • Target description
    activating transcription factor 2
  • Target synonyms
    CRE-BP1, CREB-2, CREB2, HB16, TREB7, cyclic AMP-dependent transcription factor ATF-2, cAMP response element-binding protein CRE-BP1, cAMP responsive element binding protein 2, formerly, cAMP-dependent transcription factor ATF-2, cyclic AMP-responsive element-binding protein 2, histone acetyltransferase ATF2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15336
  • Protein Name
    Cyclic AMP-dependent transcription factor ATF-2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATF2 using anti-ATF2 antibody (A00916-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF2 antigen affinity purified polyclonal antibody (Catalog # A00916-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF2 at approximately 75 kDa. The expected band size for ATF2 is at 55 kDa.
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